The most convenient sample loading and unloading in the market.
THE ONLY DIALYSIS, BUFFER EXCHANGE AND ELECTRO-ELUTION TOOL YOU’LL EVER NEED
- Floating rack and supporting trays make high throughput easy.
- Low cost single use, single step unit; no cleaning or autoclaving.
- 70% recovery of protein & over 90% recovery of DNA / RNA from PAGE
- Available in a variety of volume and MWCO options.
- Guaranteed Ultra Clean; EDTA treated and free of any contaminants.
- Now available with an Oligo PAGE purification protocol yielding over 90% Oligo recovery rate from PAGE.
*Please note that not all MWCO are available in each size of GeBAflex-tube.
See list of available GeBAflex Tube Kits below.
Specifications
Sizes
Mini {10-250 μl}
Midi {50-800 μl}
Maxi {0.1-3 ml}
Mega {3-20 ml}
MWCO
Wide range of options
1,000-50,000 MWCO
High Throughput Efficiency
Single floating rack in a single beaker can process up to
63 Mini / 35 Midi / 20 Maxi GeBAflex-tubes
Electro Elution of up to 100 samples simultanioulsy
Recovery Rate
Exceptionally High Molecule Recovery Yield:
– DNA or RNA from agarose gel – 90%
– Oligo, DNA or RNA from PAGE – 90%
– Protein from SDS-PAGE – 70%
Volume Recovery typically in the 98% range.
Guaranteed Ultra Clean
EDTA treated and free of RNase, DNase, PCR product, sulfur or heavy metal contaminants.
Sterile conditions available for custom orders.
Extended Shelf Life
12 month shelf life at room temperature
(for most models, see storage conditions for more details).
Ease of Use
secure and convenient loading & unloading with standard pipette tip, surpasses any other product in the market!
Single step, single use and low cost.
Reduced human error and contamination – no need for cleaning and autoclaving.
GeBAflex-tube caps option improves dialysis effect
by maximizing liquid surface membrane contact.
Applications
Dialysis & Buffer Exchange
High throughput Dialysis or buffer exchange
peptide dialysis small as 10 amino acids
pH and buffer adjustment of sample solution, protein extract or cell extract
Large scale protein dialysis such as antibodies and recombinant protein purification
Sample concentration
virus particles purification
Removal of contaminating micro molecules
Tissue culture extract purification
Removal of salts, surfactants, ethidiumbromide, urea, solvents and detergents
Molecule Extraction
Preparation of protein samples for MALDI-MS.
Complex formation studies (protein-protein, protein-DNA and protein-RNA)
Extraction of proteins, RNA, DNA or oligos (as small as 15 nt up to over 10kb) from polyacrylamide, agrose or any gel matrix in any running buffer.
Extraction of protein-protein, DNA-protein or RNA-protein complexes.
2D gel dots extraction.
High throughput Electro elution from 1D and 2D gel (supporting tray fits most horizontal electrophoresis units on the market).
Additional Information
Storage Conditions
GeBAflex-tube kit must be stored in a dry place at room temperature (15-25°C). Under these conditions, GeBAflex-tube kit can be stored for up to 12 months without any deterioration in performance and quality. For longer storage time, it is recommended that the GeBAflex-tube kit be stored in a cool place (refrigerator), at relative humidity of 35% at least. GeBAflex Tubes with MWCO of 1,000 / 25,000 / 50,000 are delivered wet and must be stored at 4°C.
Product Use Limitations
GeBAflex-tube kit is developed, designed and sold for research purposes only. It is not to be used for human diagnostic purposes or drug production nor for producing any substance intended to be administered to humans unless expressly cleared for that purpose by the Food and Drug Administration in the USA or the appropriate regulatory authorities in the country of use. All due care and attention should be exercised in the handling of materials described in this text.
Quality Control
The performance of GeBAflex-tube kit is regularly monitored. GeBAflex-tube kit is tested by using it for extraction of Proteins, DNA and RNA fragments of various sizes from either agarose or polyacrylamide gel. GeBAflex-tube kit is tested also for simple dialysis of salts or buffer exchange. The quality of the isolated Protein, DNA and RNA fragments or of the sample after dialysis is checked by several assays commonly used for proteins, nucleic acids and dialysis. Determining the recovery from a specific amount of loaded sample tests the quality and efficiency of the GeBAflex-tube membrane.
GeBAflex Tube Kits
Mini
| Size | MWCO | Units in Kit | Catalogue# |
|---|---|---|---|
| Mini GeBAflex-tube | 6,000-8,000 | 10 | DO70-6-10 |
| Mini GeBAflex-tube | 6,000-8,000 | 30 | DO70-6-30 |
| Mini GeBAflex-tube | 12,000-14,000 | 10 | DO70-12-10 |
| Mini GeBAflex-tube | 12,000-14,000 | 30 | DO70-12-30 |
| Mini GeBAflex-tube | 25,000 | 5 | D070-25-5 |
Midi
| Size | MWCO | Units in Kit | Catalogue# |
|---|---|---|---|
| Midi GeBAflex-tube | 1,000 | 5 | DO15-1-5 |
| Midi GeBAflex-tube | 3,500 | 10 | DO10 |
| Midi GeBAflex-tube | 3,500 | 30 | D012 |
| Midi GeBAflex-tube | 6,000-8,000 | 30 | DO20 |
| Midi GeBAflex-tube | 6,000-8,000 | 10 | DO22 |
Maxi
| Size | MWCO | Units in Kit | Catalogue# |
|---|---|---|---|
| Maxi GeBAflex-tube | 3,500 | 5 | DO30 |
| Maxi GeBAflex-tube | 3,500 | 15 | D035 |
| Maxi GeBAflex-tube | 6,000-8,000 | 5 | DO40 |
| Maxi GeBAflex-tube | 6,000-8,000 | 15 | DO45 |
| Maxi GeBAflex-tube | 12,000-14,000 | 5 | DO50 |
| Maxi GeBAflex-tube | 12,000-14,000 | 15 | DO55 |
| Maxi GeBAflex-tube | 25,000 | 5 | D060-25-5 |
| Maxi GeBAflex-tube | 50,000 | 5 | D060-50-5 |
Mega
| Size | MWCO | Cap Size | Units in Kit | Catalogue# |
|---|---|---|---|---|
| Mega GeBAflex-tube | 3,500 | 20ml | 10 | DO80-3,5/20-10 |
| Mega GeBAflex-tube | 3,500 | 15ml | 10 | DO80-3,5/15-10 |
| Mega GeBAflex-tube | 3,500 | 10ml | 10 | DO80-3,5/10-10 |
| Mega GeBAflex-tube | 6,000-8,000 | 20ml | 10 | DO80-6/20-10 |
| Mega GeBAflex-tube | 6,000-8,000 | 15ml | 10 | DO80-6/15-10 |
| Mega GeBAflex-tube | 6,000-8,000 | 10ml | 10 | DO80-6/10-10 |
| Mega GeBAflex-tube | 12,000-14,000 | 20ml | 10 | DO80-12/20-10 |
| Mega GeBAflex-tube | 12,000-14,000 | 15ml | 10 | DO80-12/15-10 |
| Mega GeBAflex-tube | 12,000-14,000 | 10ml | 10 | DO80-12/10-10 |
| Mega GeBAflex-tube | 1,000 | 20ml | 5 | D080-1/20–5 |
| Mega GeBAflex-tube | 1,000 | 15ml | 5 | D080-1/15–5 |
| Mega GeBAflex-tube | 1,000 | 10ml | 5 | D080-1/10–5 |